A few weeks back I upgraded to the latest Printrun and Slic3r and had a lot of issues popup on my AO-100 since. I narrowed down the majority of the issues to Printrun, many of which appears to be fixed by Kliment in his latest commit.
I am still having issues with the latest Slic3r though. The version I used before this was the one that came before they switched to individual setting save files. While I have things slicing correctly, and the prints turn out fine, when I start a print job and the motors activate, the Z Axis will try to lower itself even further for about 30ms then raise back up the the zero position and continue on with the print. I have my endstop adjusted correctly, and in fact, it works fine if I run using the old Slic3r / pronterface combo. To eliminate a printrun / slic3er incompatibility, I installed Repetier Host and am experiencing the same issue, so I am convinced that the problem lies within an incorrect setting in Slic3r. Has anyone else experienced this?